This is a series of technologies developed by RIKEN for preparing full-length cDNA. Extension, selection, normalization, and new cloning vectors have been developed and are being applied in many fields. Used in FANTOM1,2 and 3.
A new gene expression technique adapted for single molecule sequencing to accurately and quantitatively measure gene expression levels using only 100 nanograms of total RNA. Used in FANTOM5.
This is a simplified protocol compatible with Illumina HiSeq2500 that further increases the accuracy and depth of the conventional CAGE method, mainly used in FANTOM6 phase I and phase II.