Protocols:HeliScopeCAGE: Difference between revisions
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*Manual library preparation (standard): OP-HELISCOPE-CAGE-v3.12 | *Manual library preparation (standard): OP-HELISCOPE-CAGE-v3.12 | ||
*Automated library preparation: OP-HELISCOPE-CAGE-v4.0 | *Automated library preparation: OP-HELISCOPE-CAGE-v4.0 | ||
*HeliScope sequencing: OP-HELISCOPE-sequencing-v1.0 | *HeliScope sequencing: OP-HELISCOPE-sequencing-v1.0 | ||
*Filtering with HeliSphere/filterSMS: OP-HELICOS-CAGE-Filtering-v1.0 | *Filtering with HeliSphere/filterSMS: OP-HELICOS-CAGE-Filtering-v1.0 | ||
Revision as of 12:04, 30 August 2012
We started HeliScope CAGE with manual operation (with multi(8)-channel pipet), and achieved also automated preparation of the CAGE libraries to scale up the number of profiles (96 RNAs can be treated at once).
HeliScopeCAGE library construction
- Manual library preparation (standard): OP-HELISCOPE-CAGE-v3.12
- Automated library preparation: OP-HELISCOPE-CAGE-v4.0
- HeliScope sequencing: OP-HELISCOPE-sequencing-v1.0
- Filtering with HeliSphere/filterSMS: OP-HELICOS-CAGE-Filtering-v1.0
