Protocols:HeliScopeCAGE

We started HeliScope CAGE with manual operation (with multi(8)-channel pipet), and achieved also automated preparation of the CAGE libraries to scale up the number of profiles (96 RNAs can be treated at once).

HeliScopeCAGE library construction

• Manual library preparation (standard): OP-HELISCOPE-CAGE-v3.12
• Automated library preparation: OP-HELISCOPE-CAGE-v4.0
• HeliScope sequencing: OP-HELISCOPE-sequencing-v1.0
• Filtering with HeliSphere/filterSMS: OP-HELICOS-CAGE-Filtering-v1.0